Checklist for Preventive Maintenance of HPLC

A preventive maintenance checklist is a set of written tasks that guide the technician through preventive maintenance before it can be closed. A checklist gets all the steps and information out of a manual and into the hands of experienced technicians.

HPLC Preventative Maintenance Checklist

Customer name:____________________

Address:__________________________

________________________________

Instrument serial number: ______________

Instrument ID: ______________________

Date: ____________________________

1. Tick (check) each box as the steps are completed.
2. Cross (strike out) each box if the step cannot be completed successfully or a condition is rectified.
3. Mark "NA" for any step that is not applicable.
4. Reset the counter and date for any consumable part that is replaced, by selecting the desired part replaced action.
5. Run the appropriate Service test for any module undergoing replacements of major components.

ALSO READ: SOP for Preventive Maintenance of HPLC

Initial Performance Evaluation

⬜    Conduct a System Test in the software, and ensure it passes. Print the results and store a copy on the PC.

Leak Sensor on the Leak Tray

⬜   Enable the leak sensor on the leak tray by checking the "enable" leak sensor box in the system parameters page. The action should be "stop pumps immediately".

⬜   Start the Pumps and spill enough water in the area of the leak sensor to see it pour down the drain tube. Ensure that the pumps go offline.

Analytical Flows Module

⬜ Check the inlet and outlet check valve lifetime counter and change the check valves if needed.

⬜ Check the number of hours on the Piston Seal in the counter page and replace if necessary.

⬜ Check the number of hours on the Drain Valve Seal and Drain Valve Filter and replace if required.

⬜Check the number of hours on the Solenoid Valve and replace if needed.

⬜ Check the number of hours on the Mixer and Solvent filters and replace if required.

⬜ Check the number of hours on the 4-way joint and replace if required.

Semi-Prep/Prep Flows Module

⬜    Check the number of hours on the Piston Seal (A, B, and injector pump) in the counter page for each pump in the system, and replace if necessary.

⬜   Check the inlet and outlet check valve lifetime counter for each pump in the system (A, B, and injector) and change the check valves if needed.

⬜   Check the number of hours on the 1.2 ml SS mixer filter and replace if required. Also replace the magnetic stirrer if it looks damaged

ALSO READ: Use and Benefits of HPLC Guard Column

⬜ Check the number of hours on the solvent filter and replace if required.

Autosampler Module

⬜ Check the number of strokes on the syringe and replace if required.

⬜Check the number of movements since the injector port seal was replaced and replace if required.

⬜Check the number of movements for the injection valve stator and rotor and replace if required.

⬜Check the number of movements for the syringe valve stator and rotor and replace if required.

Manual Injector Module

⬜Check the number of switches on the column valve rotor and stator and replace if required.

⬜ Check the number of switches on the column valve stator and replace if required

Column Heater and Switching Valve Module

⬜ Check the number of switches on the column valve rotor and stator and replace if required.

⬜   Set the column temperature in the status panel, turn the oven on and read back the temperature.

PDA/UV-Vis Detector Module

⬜ Check the number of D2 (UV) lamp ignitions and running lamp hours. Replace the lamp if necessary and run a lamp calibration.

⬜Check the number of Quartz Halogen (Vis) lamp ignitions and running lamp hours. Replace the lamp if necessary and run a lamp calibration.

⬜Check the number of running hours on the flow cell and clean if necessary

⬜ For the UV/Vis detector check the percentage of D2 Lamp intensity loss in the counter page and replace if required

⬜ Under the service test tab in software run a wave scan test and check to make sure that the lamp counts for the D2 lamp at maxima are above 2000 counts. If they are not, then replace the D2 lamp, clean the flow cell and try it again.

ALSO READ: SOP for Routine Maintenance of the Dionex HPLC

RI Detector Module

⬜  Check the number of running hours on the LED and replace if required.

⬜  Clean the flow cell. If replacing the flow cell, ensure that both flow cell seals are also replaced.

ELSD Module

⬜ Check the number of running hours on the LED and replace if required.

⬜ Check the number of hours run since the last Evaporator Tube cleaning and clean the evaporator tube if necessary.

FL Module

⬜ Check the number of running hours on the Xenon lamp, and replace the lamp if required. Remember to run a re-alignment of the lamp if it is replaced.

⬜  Check the number of running hours on the Hg lamp, and replace this lamp if required. No final alignment is required for this lamp.

⬜  Check the number of running hours since the last flow cell clean and clean or replace as necessary.

Scale Up Module

⬜  Check the number of switches on the column valve rotor and stator and replace if required.

⬜  Check the number of switches on the column valve stator and replace if required.

ALSO READ: Daily and Routine LC Maintenance

Fraction Collector Module

⬜ Check the number of switches on the solenoid valve and replace if necessary.

⬜ Check the number of hours run since the last Evaporator Tube cleaning and clean the evaporator tube if necessary.

Instrument and environmental conditions

Good Fair Poor Room temperature: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Comments and recommendations

____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Signatures

Customer: ___________                    Technician: ___________

ALSO READ: What is the Expiry Date for HPLC Mobile Phase?