1. Ethylene Glycol
IUPAC Name: Ethane-1,2-diol
CAS Number: 107-21-1
Molecular Weight: 64.053 g/mol
Molecular Formula: C2H6O2
2. Diethylene Glycol
IUPAC Name: 2-(2-hydroxyethoxy) ethanol
CAS Number: 111-46-6
Molecular Weight: 106.12
Molecular Formula: C4H10O3
Determination of Diethylene Glycol & Ethylene Glycol
Diluent: Acetone (GC Grade)
Impurity stock solution (Ethylene glycol): Weight accurately 100.0mg of ethylene glycol impurity transfer in to a 100 ml volumetric flask and volume with acetone and filter.
Impurity stock solution (Diethylene glycol): Weight accurately 100.0mg of diethylene glycol impurity transfer in to a 100 ml volumetric flask and volume with acetone and filter.
Impurity Standard Solution: 5 ml of each resulting solution dilute to 100 ml volume with acetone.
Test Solution: Weight accurately transfer about 5.88g or 5ml of sample in to 100 ml volumetric flask volume with acetone and mix properly. And filter.
Chromatography System
Mode: Gas chromatography
Detector: Flame Ionization
Column: 0.53 mm x 30 m Fused-silica analytical column coated with 1.8µm; DB-624 or any equivalent.
Injector Temperature: 220°C
Detector Temperature: 250°C
|
Initial Temperature |
Temperature Ramp (áµ’/min) |
Final Temperature (áµ’C) |
Hold Time at Final Temperature (min) |
|
60 |
- |
60 |
4 |
|
60 |
50 |
120 |
10 |
|
120 |
50 |
220 |
6 |
Column: See the temperature program table
Carrier gas: Nitrogen
Injection size: 1.0µl
Flow Rate: 4.5 ml/min
Spilt Ratio: 10:1
Batch Table
|
Sr. No |
Name of Injection |
Injection No |
Injection Volume |
|
1 |
Blank
Solution |
01 |
1 µl |
|
2 |
Impurity Standard
–Ethylene Glycol solution & Diethylene Glycol solution |
05 |
1 µl |
|
3 |
Test
Solution |
02 |
1 µl |
System Suitability requirement
System Suitability solution: Impurity Standard Solution
Relative Standard Deviation: Not more than 10% Ethylene Glycol solution & Diethylene Glycol solution
Calculation
Limit:
Not more than 0.1 % of Ethylene glycol & Not more than 0.1% of Diethylene glycol
