Questions and Answers for HPLC

Which of the following is not true about High-pressure liquid chromatography (HPLC)?

1. It requires high pressure for the separation of the specious
2. There is no need to vaporize the samples
3. It is performed in columns
4. It has high sensitivity

High-pressure liquid chromatography can be performed only in columns.

1. True
2. False

Which of the following is not an advantage of Syringe-type pumps used in High-pressure liquid chromatography?

1. Independent of viscosity
2. Pulse-less flow
3. High-pressure capability
4. Unlimited solvent capacity

Which of the following is not true about solvent programming which is done in high-performance liquid chromatography?

1. It provides unequal bandwidth.
2. It provides fast overall separation.
3. It provides maximum resolution.
4. It provides maximum sensitivity.

Which of the following pulse dampers takes up some amount of the pulsation energy that is released to provide smooth pressure without pulsations?

1. Flexible bellows or compressible gas passed through tee columns
2. Flexible inert diaphragm
3. Electronic pulse damper
4. Electrical pulse damper

Which of the following is not a characteristic of the syringe pump used in high-pressure liquid chromatography?

1. Pressure capability is high
2. Maintenance is frequent
3. Limited reservoir capability
4. Slight change in flow rate when extremely high pressure compresses the solvent

Syringe pumps used in High-pressure liquid chromatography are most suitable for which of the following columns?

1. Capillary columns
2. Guard columns
3. Short-fast columns
4. Small bore columns

ALSO READ: How to Select Buffer in HPLC Method Development?

The gravity feed method for solvent delivery is not used with narrow bore columns packed with fine mesh particles.

1. True
2. False

Which of the following cannot be done to reduce ripple in High-pressure liquid chromatography?

1. Using bellows
2. Using restrictors
3. Using long nylon tube between pump and column
4. Avoiding the use of the solvent pump

Which of the following is not true about Hydraulic capacitance flow control system used in HPLC?

1. It can be used only for liquids with low viscosity
2. It is irrespective of solvent compressibility
3. It maintains a constant flow
4. It smoothens high pressure pump pulsations

Derivatization techniques in HPLC are intended to enhance

1. Molecular weight
2. Reversibility
3. Detectability
4. Reproducibility

In HPLC, the analytical performance improves when 

1. Particle diameter in increased
2. Particle diameter is reduced
3. Coarser particles are paired with shorter columns
4. Low temperature is used

What is the nature of mobile phase in reverse phase chromatography?

1. Polar
2. Non polar
3. Mixture of both
4. Can be any it depends on the nature of analyte

Solvent programming, also called gradient elution, involves:

1. Changing the column length
2. Using the mobile phase composition
3. Successive injection of sample
4. Changing the mobile phase composition

The pressure used in HPLC is:

1. 1000-3000 psi
2. 1000-5000 psi
3. 2000 to 6000 psi
4. 1000 to 6000 psi

ALSO READ: Peak Purity and Peak Identification

An essential requirement of the mobile phase in HPLC is that:

1. It must have constant flow rate with pulses
2. It must run at 20°C only
3. It must be freshly distilled
4. It must flow with pulses

HPLC is an abbreviation for

1. High Profit Liquid Chromatography
2. High Pressure Liquid Chromatography
3. Higher Performance Low Chromatography
4. Higher Profit Low Chromatography

In reverse phase chromatography, the stationary phase is made

1. Non-polar
2. Polar
3. Either non-polar or polar
4. None of these

Derivatization techniques in HPLC are intended to enhance

1. Molecular weight
2. Detectability
3. Reversibility
4. Reproducibility

Indicate the HPLC detector that is most sensitive to change in temperature:

1. PDA detector
2. Refractive Index detector
3. Fluorescence detector
4. Electrochemical detector

Which of following HPLC detectors is not a solute property detector?

1. UV–Visible detector
2. Photo diode array detector
3. Fluorescence detector
4. Refractive index detector

Which of following HPLC detector is a bulk property detector?

1. Refractive index detector
2. UV detector
3. Photo diode array detector
4. Fluorescence detector

For amino acid analysis by HPLC derivatization reagent used by UV absorption detector is

1. 4-Nitrobenzyl-N-propylamine hydrochloride
2. 4-Nitrobenzyloxyamine hydrochloride
3. 3,5-Dinitro benzoyl chloride
4. 4-Nitrobenzyl-N-N’-disopropylisourea

In HPLC the analytical performance improves when

1. Particle diameter is increased
2. Particle diameter is decreased
3. Coarser particles are paired with shorter columns
4. Low temperature is used

RP-HPLC method contains

1. Stationary phase is polar and mobile phase is non-Polar
2. Stationary phase is non-polar and mobile phase is polar
3. Stationary phase is non-polar and mobile Phase is non-polar
4. Stationary phase is polar and mobile phase is polar

ALSO READ: Understanding of HPLC Audit Trails

1.  2	2. 1	3. 4	4. 1	5. 1
6. 2	7. 4	8. 1	9. 4	10. 1
11. 3	12. 2	13. 1	14. 4	15. 4
16. 1	17. 2	18. 1	19. 2	20. 2
21. 4	22. 1	23. 3	24. 2	25. 2