Chromatography Basic Principles: Sample Preparations and Related Methods

The Handbook of Chromatography: Basic Principles, Sample Preparations, and Related Methods is a comprehensive reference that gives practical information on chromatography's principles, techniques, and applications. This guide was written by a group of experienced chromatography scientists and covers a wide range of subjects, from the principles of chromatography to advanced methods and applications.


The first portion of the handbook presents the fundamental concepts of chromatography, such as the many forms of chromatography, the variables that influence chromatographic separation, and the chromatographic instruments. This section offers practical advice and ideas on how to maximize chromatographic separation, including column, mobile phase, and gradient conditions selection.

The second section of the handbook focuses on the practical aspects of sample preparation for chromatographic analysis. This section covers the different techniques for sample preparation, including extraction, filtration, and derivatization, as well as the different detection methods, including UV, fluorescence, and mass spectrometry. It also provides guidelines for selecting appropriate validation parameters, such as accuracy, precision, linearity, and sensitivity.


The handbook's third part discusses advanced chromatography techniques and applications, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and supercritical fluid chromatography (SFC). In addition, this part offers case studies and illustrations of how chromatography may be utilized in a variety of applications, such as environmental monitoring, food analysis, and pharmaceutical research. 

Overall, the Handbook of Chromatography: Basic Principles, Sample Preparations, and Related Methods is a valuable reference for chromatographers. It presents a comprehensive explanation of chromatography's concepts, methodologies, and applications, as well as practical guidance and recommendations that may be applied to a wide range of analytical difficulties in a variety of sectors.

Finally a book on chromatography that is easy to grasp for undergraduates and technicians; covers the area in sufficient depth while still being concise. The book includes all recent technology advances and has core textbook features further improving the learning experience.

This book is the perfect introduction to a methodology that is the underlying principle of the vast majority of separation methods worldwide. Everyone working in a lab environment must be familiar with the basis of these technologies and Tyge Greibrokk, Elsa Lundanes, and Leon Reubsaet succeed in delivering a text which is easy to read for undergraduates and laboratory technicians and covers the area in sufficient depth while still being concise. The book includes all recent technology advances and has core textbook features further improving the learning experience.

Importantly, the text does not only cover all major modern chromatography technology (thin layer, gas, high-pressure liquid, and supercritical fluid chromatography) but also related methods, in particular electrophoretic technologies.



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