Chromatography

Through informative articles and tutorials, we aim to demystify the complexities of HPLC, method development, and troubleshooting. Our team are passionate about sharing their knowledge and experiences, ensuring that you gain a deep understanding of HPLC principles and their practical applications.

Calculation of System Suitability Parameters

Common Mistakes to Avoid When Using HPLC Mobile Phases

Retention Time Variability in HPLC

Diagnostics Tips and Tricks for System Pressure Problems in HPLC

What is the Difference between HPLC and UPLC?

What is the Difference between HPLC and HPTLC?

Which Solvent is Better for Chromatography: Methanol or Acetonitrile?

Troubleshooting Problems Detected by Smell, Sight or Sound in HPLC

Why Degassing of HPLC Mobile Phase is Necessary?

Troubleshooting Leaks in HPLC

General HPLC Column Care

Troubleshooting Problems with Ghost Peaks

Troubleshooting Problems with Peak Shape (Peak Tailing, Split Peaks, Peak Fronting)

Peak Purity and Peak Identification

Abnormal Peak Shapes

What is Peak Tailing?

What is LC-MS Analysis?

How to Develop HPLC Method?

Checklist for Preventive Maintenance of HPLC

How to Make HPLC Columns last Longer?

Chromatographic Calculations

What is the Shelf Life for HPLC Columns?

What is the Expiry Date for HPLC Mobile Phase?

What is HPLC Column Conditioning? How Long to Equilibrate?

Do's and Don'ts: HPLC Analysis

Maintenance of Flow Cell

Daily and Routine LC Maintenance

Causes and Remedy for HPLC Troubleshooting

How to Select Buffer in HPLC Method Development?

Troubleshooting for UV Detector of HPLC

Column Restoration Procedures

HPLC Column Care and Maintenance

What is the Difference between C8 and C18 columns in HPLC?

Requirements for Analytical Method Development and Validation for HPLC

Use and Benefits of HPLC Guard Column

Water Used for HPLC Analysis

Troubleshooting Problems with Column Backpressure

What is the RSD Limit for Replicate Standards in HPLC Analysis?